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應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離:一種新的技術(shù)方法——結(jié)論、致謝!

來源:上海謂載 瀏覽 1047 次 發(fā)布時間:2021-12-20

5.結(jié)論


首先,表面張力測量證明牛精漿具有很好的表面活性,可能與BSP蛋白有關(guān)。其次,Langmuir膜法(先前被證明是公牛精子外細胞膜的相關(guān)脂質(zhì)單層模型[35])有助于表征BSP蛋白對脂質(zhì)膜的親和力。BSP蛋白能夠到達被磷脂覆蓋的牛精子細胞表面,這得益于它們與磷脂酰膽堿的相互作用和自身的表面活性。我們的假設是,由于它們自身的表面活性,它們首先穿透精子的外小葉,然后由于與磷脂酰膽堿的強烈相互作用而留在那里。最后,利用Langmuir膜法對已知的BSP蛋白螯合劑如ldl和脂質(zhì)體的作用進行了篩選。脂質(zhì)體與低密度脂蛋白一樣有效地阻止BSP蛋白插入磷脂層。低密度脂蛋白和脂質(zhì)體的比例相同:0.16–0.17 mg磷脂酰膽堿/mg BSP。我們還不知道在冷凍保存的時間尺度上脂質(zhì)體的非常微弱的插入是否有一些生物學后果。需要進一步研究分析每種BSP蛋白的表面性質(zhì)及其在膜上的作用。此外,可以測試Langmuir膜,以檢測BSP與膜的親和力隨溫度的變化[20]。最后,Langmuir膜法是一種有效的篩選BSP蛋白質(zhì)新螯合劑的方法。該方法可適用于其他動物物種。


致謝


作者感謝IMV Technologies(法國)的財務支持,Gérard Chatagnon負責精漿蛋白質(zhì)定量,Véronique Solé負責凝膠電泳,Maureen Collobert負責英語校正,Alain Sire和Patrice Papineau負責手套箱的構(gòu)思和實現(xiàn),允許在受控大氣中使用朗繆爾槽。


附錄A.補充數(shù)據(jù)


與本文相關(guān)的補充數(shù)據(jù)可在在線版本中找到,網(wǎng)址為http://dx.doi.org/10.1016/j.colsurfb.2015.11.34


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